Lung Epithelial Cell Line A549

Lieber m et al.

Lung epithelial cell line a549.

Cell culture and seeding of a549. The a549 cell line was established in 1972 by d j. The a549 cell line is a suitable host for many human. Cell culture nsclc cells a549 nci h23 nci h1299 and hcc827 and human normal lung epithelial cells beas 2b were bought from american type culture collection atcc manassas va usa.

The cell line of a549 was cultured in atcc formulated modified f 12 k medium containing 10 fetal bovine serum 100 u ml penicillin and 100 u ml streptomycin sulfate. Application enzymatic studies human a549 cell line has been. The a549 cell line was isolated in 1973 from a pulmonary adenocarcinoma. Lung cancer and normal cell lines.

A549 cells were cultured in kaighn s modification of ham s f 12 f 12k medium atcc nci h23 nci h1299 and hcc827 cells were cultured in rpmi 1640. A549 a cell line model of nsclc used to evaluate the role of ccl22 and il 37 in epithelial mesenchymal transition 3 toxicology studies cellular toxicity of carbon based nanomaterials was tested in calu 1 and other lung cancer cell lines 4. Small cell lung cancer homo sapiens 59 years caucasian the cells do not express vasopressin oxytocin. A549 cell line origin.

Cross references nucleotide genbank. Human caucasian lung carcinoma cell line description derived from a 58 year old caucasian male. 20 mm and 10 fetal calf serum at 37 c. The cells are used as models for the study of lung cancer and the development of drug therapies against it.

A549 cells are hypotriploid alveolar basal epithelial cells. A549 cells are adenocarcinomic human alveolar basal epithelial cells and constitute a cell line that was first developed in 1972 by d. This adenocarcinomic cell line is categorized as a non small cell lung carcinoma nsclc which tends to. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway.

In a study to. X54079 human mrna for heat shock protein hsp27. Giard et al through an explant culture of adenocarcinomic lung tissue of a 58 year old caucasian male. Through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58 year old caucasian male.

Cells from the human lung epithelial carcinoma cell line a549 atcc ccl 185 were maintained in dulbecco s eagle s medium supplemented with penicillin 0 6 μg ml 1 streptomycin 60 μg ml 1 glutamine 2 mm hepes buffer ph 7 4. Human lung epithelial cells a549 which were bought from shanghai institutes for biological sciences. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.